What email address or phone number would you like to use to sign in to Docs.com?
If you already have an account that you use with Office or other Microsoft services, enter it here.
Or sign in with:
Signing in allows you to download and like content, and it provides the authors analytical data about your interactions with their content.
Embed code for: OneDriveOneDriveHow Different pH Levels Effect Bacteria Growth
Select a size
How Different pH Levels Effect Bacteria Growth
By: Niles Boehm
In an acidic environment, bacteria growth will be of a lesser value rather than in an environment of a base solution.
The above hypothesis of how different pH levels effect bacteria growth will be tested as an experiment. It will be tested to see if an acidic environment is more likely to reduce bacteria growth rather than a basic environment. In terms of the relevance for this experiment, it will be done to test which method is more effective in limiting bacteria growth. These experiments have been done many times for research on anti-bacterial agents such as house cleaning products. This shows relevance to outside research within this certain experiment.
Acids and Bases
In this experiment, multiple ranges of acids and bases will be tested. Acids ranging from pH levels 1-6, a neutral pH level being 7, and bases ranging from 8-14.
Acids and Bases
Both the acids and bases that will be used are common. The acid that will be used is Hydrochloric Acid (HCl), and the base that will be used is Sodium Hydroxide (NaOH). The neutral substance that will be used is purified water.
Types of Bacteria
Four types of bacteria will be used. Two beneficial and two harmful. The bacteria will grow in a broth mixture.
The two beneficial bacteria that will be used are Streptococcus Thermophilus, and Lactobacillus Acidophilus.
The two harmful bacteria that will be used are Escherichia Coli and Salmonella Typhi.
To maintain a constant temperature an incubator will be used. The constant temperature will be 37 degrees Celsius. While measuring the growth, the bacteria will be taken out of the incubator just long enough for measurements.
In order for the bacteria to grow, a nutrient rich atmosphere must be present. In this experiment, as stated previously, a broth mixture will be used.
In order to count the bacteria effectively, bacteria growth will be measured every 24 hours. The reason for this time period is because an initial bacteria culture takes around 24 hours to grow. 24 hours gives a more efficient time period to allow growth.
The bacteria cultures will be measured by means of Turbidimetric Measurement. In this type of measurement, Turbidity of a culture is measured. The turbidity is then used to figure out the cell count in a culture. Turbidity is the cloudiness or haziness of a fluid caused by individual particles.
This method is much faster than other methods but can become complex. With different measurements of the bacteria cultures, initial cell numbers must be calculated.
The turbidity is then used to determine the amount of viable samples per culture. The average of this is then used to draw a curve, which then gives the amount of bacteria per culture.
Turbidity is measured by using a Spectrophotometer. This piece of equipment uses light to pass through the cell. Light not being able to pass through the cell shows if cells are present. If one cell blocks the ability of light to detect another cell, measurements are no longer valid.
http://www.newhealthguide.org/Types-Of-Bacteria.htmlhttp://www.newhealthguide.org/Types-Of-Bacteria.html (New Health Guide 2016) (Date Accessed September 14, 2016)
http://www.aqion.de/site/191http://www.aqion.de/site/191 (Aquion 2016) (Date Accessed September 14, 2016)
http://aem.asm.org/content/39/3/604.shorthttp://aem.asm.org/content/39/3/604.short (Russel and Dombrowski 1980) (Date accessed September 14, 2016)
http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=2&SID=4AvmvNMIHh2TUe5Gm3R&page=1&doc=9http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=2&SID=4AvmvNMIHh2TUe5Gm3R&page=1&doc=9 (Cuadra, Frantellizzi, Gaesser, Tammariello, Ahmed 2016) (Date accessed September 15, 2016)
http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=3&SID=4AvmvNMIHh2TUe5Gm3R&page=2&doc=14http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=3&SID=4AvmvNMIHh2TUe5Gm3R&page=2&doc=14 (Tsz Wai, Li, Chow, Wong 2016) (Date accessed September 15, 2016)
http://or.water.usgs.gov/grapher/fnu.htmlhttp://or.water.usgs.gov/grapher/fnu.html (US Department of the Interior, US Geological Survery 2013) (Date accessed September 18, 2016)effective in limiting bacteria growth. These experiments have been done many times for research on anti-bacterial agents such as house cleaning products. This shows relevance to outside research within this certain experiment.